1 Crystal Structure of Mammalian 1 , 6 - Fucosyltransferase , FUT 8
نویسندگان
چکیده
Department of Disease Glycomics, Research Institute for Microbial Diseases, Osaka University, Taniguchi Research Group, 4th Flr, Center for Advanced Science & Innovation, Osaka University, 2-1, Yamadaoka Suita, Osaka 565-0871, Japan Division of Molecular Cell Biology, Department of Biomolecular, Sciences, Saga University Medical School, 5-1-1 Nabeshima, Saga 849-8501, Japan CREST, JST Graduate School of Pharmaceutical Sciences, University of Tokyo, Hongo 7-3-1, Tokyo, Bunkyo-ku, 113-0033, Japan Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan Department of Biochemistry, Osaka University Graduate School of Medicine, B1, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan Division of Regulatory Glycobiology, Institute of Molecular Biomembrane and Glycobiology, Tohoku Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai, Miyagi 981-8558, Japan Department of Molecular Genetics, Kochi University Medical School, Kohasu, Oko-cho, Nankoku, Kochi 783-8505, Japan.
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Comparisons of Caenorhabditis Fucosyltransferase Mutants Reveal a Multiplicity of Isomeric N-Glycan Structures
Recent studies have shown a remarkable degree of plasticity in the N-glycome of the model nematode Caenorhabditis elegans; ablation of glycosylation-relevant genes can result in radically altered N-glycan profiles despite only minor biological phenotypic effects. Up to four fucose residues and five different linkages of fucose are known on the N-glycans of C. elegans. Due to the complexity in t...
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Fucose is a common monosaccharide component of cell surfaces and is involved in many biological recognition events. Therefore, definition and exploitation of the specificity of the enzymes (fucosyltransferases) involved in fucosylation is a recurrent theme in modern glycosciences. Despite various studies, the specificities of many fucosyltransferases are still unknown, so new approaches are req...
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The structure of the pentasaccharide S259-1 in the Consortium for Functional Glycomics was investigated using a variety of techniques. Surprisingly, the structure differs from the structure assumed from the previously established specificity of the human fucosyltransferase FUT-III used in the last step of chemoenzymatic synthesis. When presented with a tetrasaccharide substrate containing both ...
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